Filaments and Accessories for Pipette Pullers
Box filaments are recommended for large diameter, double barreled, or aluminosilicate glass. Box filaments are particularly suitable for slice preparations where long, parallel walls would aid penetration. If using a box filament, the size of the square box should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass that you will be using. For IVF and ICSI applications, a 2.5mm x 4.5mm box filament is recommended. For pronuclear injection work, we recommend a 2.5mm x 2.5mm box filament. Call for more specific advice or to request a combination of settings, filaments, and glass for your application. Trough filaments are excellent general purpose filaments and are recommended for patch pipettes produced from either standard or thin wall glass.
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Filaments and Accessories for Pipette Pullers
Filaments
(Minimum purchase of filaments is 4)
Appropriate filament selection depends on your research application, but a general guideline for filaments is as follows:
Box Filaments – Recommended for small to large diameter borosilicate glass, double barreled, or aluminosilicate glass. Box filaments are particularly suitable for patch pipettes, slice preparations, and for sharp electrodes and microinjection pipettes where gradual parallel walls would aid cell penetration. When using a box filament, the size of the square box should be approximately 1.0mm to 1.5mm larger than the outside diameter of the glass that you will be using.
For IVF and ICSI applications, a 2.5mm x 4.5mm box filament (FB245B) is recommended. For pronuclear injection work, we recommend a 2.5 mm x 2.5 mm box filament (FB255B). Call for more specific advice or to request a combination of settings, filaments, and glass for your application.
Trough Filaments – Excellent general purpose filaments and are recommended for creating longer slice patch pipettes and sharp electrodes. The trough filament is not ideal for making patch pipettes or when using glass over 1.2 mm OD. If very short tapers are required for patch electrodes or for microinjection, a 2.5 mm or 3 mm box filament is recommended.
Custom Filaments – A custom filament can be created if your pipette needs can not be met by one of the standard box or trough filaments. Please call Sutter for details.
Sutter pre-programs the P-97 with a 2.5 mm x 2.5 mm box filament unless an alternative filament is requested.
For either shape of filament (box or trough), increasing the filament width tends to increase the length of the pipette taper. If there are specific questions as to the sizes needed, please call and our technical support staff will guide you in your choice.
Ordering Information
Box Filaments |
|
| Catalog Number | Description |
| FB215B | 2.0 mm square box filament, 1.5 mm wide |
| FB220B | 2.0 mm square box filament, 2.0 mm wide |
| FB230B | 2.0 mm square box filament, 3.0 mm wide |
| FB255B | 2.5 mm square box filament, 2.5 mm wide |
| FB245B1 | 2.5 mm square box filament, 4.5 mm wide |
| FB315B | 3.0 mm square box filament, 1.5 mm wide |
| FB320B | 3.0 mm square box filament, 2.0 mm wide |
| FB330B | 3.0 mm square box filament, 3.0 mm wide |
Trough Filaments |
|
| Catalog Number | Description |
| FT315B | 1.5 mm wide trough filament |
| FT320B | 2.0 mm wide trough filament |
| FT330B | 3.0 mm wide trough filament |
| FT345B | 4.5 mm wide trough filament |
Custom Filaments |
|
| Catalog Number | Description |
| FILAMENT | Custom platinum/iridium filament (Phone Sutter for details) |
P-30 Filaments |
|
| Catalog Number | Description |
| PF30T15 | 1.5 mm wide trough filament |
| PF30T20 | 2.0 mm wide trough filament |
| PF30T30 | 3.0 mm wide trough filament |
| PF30N2 | Nichrome filament – 3 turns (BEFORE SN# P30-680) |
| PF30N-42 | Nichrome filament – 4 turns (AFTER SN# P30-679) |
P-77A Loop Filaments |
|
| Catalog Number | Description |
| (SN# 160 and greater) | |
| FL315A | 3.0 mm loop filament, 1.5 mm wide |
| FL320A | 3.0 mm loop filament, 2.0 mm wide |
| FL325A | 3.0 mm loop filament, 2.5 mm wide |
| (SN# less than 160) | |
| FL315X | 3.0 mm loop filament, 1.5 mm wide |
| FL320X | 3.0 mm loop filament, 2.0 mm wide |
| FL325X | 3.0 mm loop filament, 2.5 mm wide |
Accessories
| Catalog Number | Description |
| FPS | Fire polishing spacer for P-97, P-87, P-2000 pullers |
| FS1875 | Platinum/iridium sheet, 18 mm x 75 mm x 0.05 mm (0.002 in) |
| CTS | Ceramic tile for scoring glass |
| BX10 | Pipette storage box – Holds 10 (4 3/4 x 3 5/8 x 3/4 in) |
| BX20 | Pipette storage box – Holds 20 (7 x 3 5/8 x 3/4 in) |
1 For P-87, please contact Sutter
2 P-30/N pullers (serial number lower than P-30-680) came with a P30N filament installed. You can now select either the P30N or P30N-4 filament. With the P30N-4 you can 1) use lower heat settings, 2) pull longer tapers (2-3cm) and 3) use aluminosilicate glass. If you need to pull very short tapers, the P30N is recommended.
