Pipette Cookbook

The Pipette Cookbook is and invaluable resource for anyone pulling pipettes, electrodes and needles with Sutter Pullers.

Click here to download the Pipette Cookbook (Adobe PDF format).

Table Of Contents

• Introduction
• Using the Pipette Cookbook with the P-87 and P-80/PC
• Using the Pipette Cookbook with the P-1000
• Sutter Capillary Glass
• Sutter Filaments
• Trough Filament Shape & Alignment
• Box Filament Alignment
• Eccentric Adjustments
• Installing a Filament – Step by Step
• Chapter 1 Electrophysiology Overview
• Extracellular Recording Electrodes
• Intracellular Recording Electrodes
• Patch Pipettes
• Chapter 2 Adherent Cell, C.elegans, Drosophila, & Zebrafish
• Chapter 3 Bee-Stinger Needle
• Chapter 4 Pronuclear Injection
• Chapter 5 Embryo Stem Cell (ES Cell)
• Chapter 6 Intracytoplasmic Sperm Injection (ICSI)
• Chapter 7 Nuclear Transfer (NT)
• Chapter 8 Holding Pipettes
• Chapter 9 Xenopus Oocyte Injection
• Chapter 10 Large Pipettes with a 20μ to 200μ Tip & Using the Ceramic Tile (CTS)
• Chapter 11 Aspiration Pipettes:
• Blastomere Biopsy, Cell transfer, & Sinngle Cell Mechanics
• Chapter 12 Ion Selective Electrodes & Amperometric Microelectrodes
• Chapter 13 Aluminosilicate Glass and Custom Programs
• Chapter 14 Ramp Test
• Chapter 15 General Guideline for Parameter Settings
• Chapter 16 Concerns with Variability
• Chapter 17 XenoWorks Microinjection System
• Chapter 18 BV-10 Beveler
• GENERAL LOOK UP TABLES
• Type A, B, C, D, & E Programs
• General Look Up Tables
• Notes