Capillary glass
Capillary glass

Glass for Pipette Fabrication

Sutter Instrument Company, in addition to the finest micropipette pullers available, offer a wide selection of high quality capillary glasses in various sizes and materials. Though there are many types and sizes of capillary glass available, Sutter have carefully selected only those that pass their criteria. Their expertise in micropipette technology assures you of precision and high quality.

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Glass for Micropipette Fabrication

Sutter Instrument Company, in addition to the finest micropipette pullers available, offers a wide selection of high quality capillary glasses in various sizes and materials. Though there are many types and sizes of capillary glass available, we have carefully selected only those that pass our criteria. Our expertise in micropipette technology assures you of precision and high quality.

We offer capillary glass tubing in three different compositions; quartz, borosilicate and aluminosilicate. Each composition has its own unique properties and the selection will be determined by your application and your puller’s capabilities.

Fire Polishing

All borosilicate and aluminosilicate capillary glass offered by Sutter Instrument have fire-polished ends. This process eliminates any sharp edges, making it easier to insert into holders, and does not affect the electrical or mechanical properties of the glass.

Custom Pipettes

Sutter Instrument can make custom pipettes and microtools not commonly available from other pipette manufacturers. For example, we make custom pre-pulled beveled pipettes for microinjection. The custom pipettes are considered non-sterile and are manufactured for research applications and non-human use. Please contact Sutter Instrument for further details.

Sizes

Sutter Instrument capillary tubing is available in a broad range of wall thickness which allows you to select the size necessary for your application. The ratios of inside diameter to outside diameter typically run between 0.5mm and 0.75mm with the lower value being referred to as thick wall tubing and the top of the range as thin wall tubing. All other factors being equal, the thicker wall capillaries produce pipettes with longer tapers and smaller tips which make it more suitable for intracellular microelectrodes. These thicker wall pipettes tend to reduce the noise contributions due to capacitance which makes them more suitable for patch clamp pipettes. Thinner wall tubing allows for larger pore openings which make it ideal for microinjection needles and low resistance microelectrodes.

Filamented Glass – TechNote: What is filamented glass and who needs it?

Filamented glass has a small rod of glass annealed to the inner wall and this rod (filament of glass) creates the capillary action required to back-fill the pipette with solution. If the resulting pipette tip is under 1µl and being used for microinjection or being used for recording, we recommend “filamented-glass”. The filament in the glass not only provides capillary action for quick filling of the micropipette, it also helps to reduce the incidence of air bubbles when introducing solution into the pipette.

GLASS COMPOSITION

Borosilicate (CORNING 7740)

The most commonly used glass is borosilicate. Sutter Instrument offers only TYPE I-CLASS A borosilicate as described by ASTM Standard 3.1.2*. This glass softens at 821 degrees Celsius and, as it is pulled, maintains its ratio of inside diameter to outside diameter over the total taper length. Borosilicate softens at a lower temperature than our other glasses and has a wider working range. These unique properties allow for a greater variety of shapes used in microelectrodes, patch pipettes, microinjection needles and, in the case of solid rod, chromosome dissection tools.

Aluminosilicate (SCHOTT 8252)

Aluminosilicate softens at a higher temperature (935 degrees Celsius) than borosilicate and is workable over a much narrower range. It has a tendency to continuously thin out as it is drawn which allows extremely fine tips with very short tapers. For example, we have pulled aluminosilicate tips in the 20-30 nanometer range with taper lengths of 5-6 mm. Its resistivity is several orders of magnitude higher than borosilicate, thus reducing leakage currents when used in ion-selective micropipettes. Aluminosilicate is harder than borosilicate which results in a pipette that is more suitable for penetrating tough tissues.

Quartz (HERAEUS HSQ300)

The finest and purest glass available is quartz. It is superior to all other glasses in its mechanical, electrical and optical qualities. It has the lowest dielectric constant, the lowest loss factor and the highest volume resistivity making it ideal for patch clamp recording. Its chemical purity virtually eliminates leakage of ions** and by using quartz in single channel patch clamp recordings the lowest background noise levels have been achieved***. Due to its high melting point, it cannot be pulled on conventional pullers. However, it can be pulled easily with the Sutter CO2 laser-based P-2000.

* ASTM Designation E438-90 – April 1990

** Zuazaga C., Steinacker A. Patch-clamp recording of ion channels: Interfering effects of patch pipette glass. News in Physiological Sciences: International Union of Physiological Sciences and the American Physiological Society: 5:155-158, August 1990

*** Rae, James L., Levis Richard A. A Method for exceptionally low noise single channel recordings. Pflügers Archive; European Journal of Physiology:420:618-620, Springer-Verlag 1992

Thick/Standard Wall Borosilicate Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
BF100-50-7.5 1.00 mm 0.50 mm 7.5 cm 225
BF100-50-10 1.00 mm 0.50 mm 10 cm 225
BF100-50-15 1.00 mm 0.50 mm 15 cm 225
BF100-58-10 1.00 mm 0.58 mm 10 cm 250
BF100-58-15 1.00 mm 0.58 mm 15 cm 250
BF120-60-10 1.20 mm 0.60 mm 10 cm 225
BF120-69-7.5 1.20 mm 0.69 mm 7.5 cm 250
BF120-69-10 1.20 mm 0.69 mm 10 cm 250
BF120-69-15 1.20 mm 0.69 mm 15 cm 250
BF150-75-7.5 1.50 mm 0.75 mm 7.5 cm 225
BF150-75-10 1.50 mm 0.75 mm 10 cm 225
BF150-86-7.5 1.50 mm 0.86 mm 7.5 cm 250
BF150-86-10 1.50 mm 0.86 mm 10 cm 250
BF150-86-15 1.50 mm 0.86 mm 15 cm 250
BF200-100-10 2.00 mm 1.00 mm 10 cm 225
BF200-116-10 2.00 mm 1.16 mm 10 cm 250
BF200-116-15 2.00 mm 1.16 mm 15 cm 250
Thick/Standard Wall Borosilicate Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
B100-20-10* 1.00 mm 0.20 mm 10 cm 225
B100-50-10 1.00 mm 0.50 mm 10 cm 225
B100-50-15 1.00 mm 0.50 mm 15 cm 225
B100-58-10 1.00 mm 0.58 mm 10 cm 250
B100-58-15 1.00 mm 0.58 mm 15 cm 250
B114-53-10NP** 1.14 mm 0.53 mm 10 cm 225
B120-69-8 1.20 mm 0.69 mm 8 cm 250
B120-69-10 1.20 mm 0.69 mm 10 cm 250
B120-69-15 1.20 mm 0.69 mm 15 cm 250
B150-86-7.5 1.50 mm 0.86 mm 7.5 cm 250
B150-86-10 1.50 mm 0.86 mm 10 cm 250
B150-86-15 1.50 mm 0.86 mm 15 cm 250
B200-116-10 2.00 mm 1.16 mm 10 cm 250
B200-116-15 2.00 mm 1.16 mm 15 cm 250
* The ends are not fire polished. Special order minimum of 2.
** Nanoinjection glass. Ends are not fire-polished.
Heavy Polished Thick/Standard and Thin Walled Borosilicate Glass*
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
BF150-86-7.5HP 1.50 mm 0.86 mm 7.5 cm 250
BF150-86-10HP 1.50 mm 0.86 mm 10 cm 250
BF150-110-7.5HP 1.50 mm 1.10 mm 7.5 cm 225
BF150-110-10HP 1.50 mm 1.10 mm 10 cm 225
Heavy Polished Thick/Standard and Thin Walled Borosilicate Glass*
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
B100-30-7.5HP 1.00 mm 0.30 mm 7.5 cm 225
B100-50-7.5HP 1.00 mm 0.50 mm 7.5 cm 225
B150-86-7.5HP 1.50 mm 0.86 mm 7.5 cm 250
B150-86-10HP 1.50 mm 0.86 mm 10 cm 250
B150-110-7.5HP 1.50mm 1.10mm 7.5cm 225
B150-110-10HP 1.50 mm 1.10 mm 10 cm 225
*Useful for added protection of gaskets and wire in headstages.
Thin Wall Borosilicate Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
BF100-78-10 1.00 mm 0.78 mm 10 cm 250
BF100-78-15 1.00 mm 0.78 mm 15 cm 250
BF120-94-8 1.20 mm 0.94 mm 8 cm 250
BF120-94-10 1.20 mm 0.94 mm 10 cm 250
BF120-94-15 1.20 mm 0.94 mm 15 cm 250
BF150-110-7.5 1.50 mm 1.10 mm 7.5 cm 225
BF150-110-10 1.50 mm 1.10 mm 10 cm 225
BF150-117-10 1.50 mm 1.17 mm 10c m 250
BF150-117-15 1.50 mm 1.17 mm 15 cm 100
BF150-120-10 1.50 mm 1.20 mm 10 cm 250
BF165-120-7.5 1.65 mm 1.20 mm 7.5 cm 225
BF165-120-10 1.65 mm 1.20 mm 10 cm 225
BF200-156-10 2.00 mm 1.56 mm 10 cm 250
BF200-156-15 2.00 mm 1.56 mm 15 cm 100
Thin Wall Borosilicate Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
B100-75-10 1.00 mm 0.75 mm 10 cm 225
B100-75-15 1.00 mm 0.75 mm 15 cm 225
B120-90-8 1.20 mm 0.90 mm 8 cm 225
B120-90-10 1.20 mm 0.90 mm 10 cm 225
B120-90-15 1.20 mm 0.90 mm 15 cm 225
B150-110-7.5 1.50 mm 1.10 mm 7.5 cm 225
B150-110-10 1.50 mm 1.10 mm 10 cm 225
B150-117-10 1.50 mm 1.17 mm 10 cm 225
B200-156-10 2.00 mm 1.56 mm 10 cm 250
B200-156-15 2.00 mm 1.56 mm 15 cm 100
Multibarrel Borosilicate Glass*
With Filament
Catalog Number Number of Barrels Out/Inside Diameter Overall Length Pieces per Package
2BF100-50-10 2 barrels 1.00/0.50 10 cm 75
2BF100-75-10 2 barrels 1.00/0.75 10 cm 75
2BF150-86-10 2 barrels 1.50/0.86 10 cm 100
2BF150-86-15 2 barrels 1.50/0.86 15 cm 100
3BF100-50-10 3 barrels 1.00/0.50 10 cm 75
3BF100-75-10 3 barrels 1.00/0.75 10 cm 75
3BF120-69-10 3 barrels 1.20/0.69 10 cm 100
3BF120-69-15 3 barrels 1.20/0.69 15 cm 100
Multibarrel Borosilicate Glass*
Without Filament
Catalog Number Number of Barrels Out/Inside Diameter Overall Length Pieces per Package
3B100-75-10 3 barrels 1.00/0.75 10 cm 75
4B100-75-10 4 barrels 1.00/0.75 10 cm 75
* Multibarrel borosilicate requires a custom filament.
Please contact Sutter Instrument for more information when ordering.
Quartz Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
QF100-50-7.5 1.00 mm 0.50 mm 7.5 cm 100
QF100-50-10 1.00 mm 0.50 mm 10 cm 100
QF100-60-7.5 1.00 mm 0.60 mm 7.5 cm 100
QF100-60-10 1.00 mm 0.60 mm 10 cm 100
QF100-70-7.5 1.00 mm 0.70 mm 7.5 cm 100
QF100-70-10 1.00 mm 0.70 mm 10 cm 100
QF100-70-15 1.00 mm 0.70 mm 15 cm 100
QF120-60-7.5 1.20 mm 0.60 mm 7.5 cm 100
QF120-60-10 1.20 mm 0.60 mm 10 cm 100
QF120-90-10 1.20 mm 0.90 mm 10 cm 100
QF150-75-7.5 1.50 mm 0.75 mm 7.5 cm 100
QF150-75-10 1.50 mm 0.75 mm 10 cm 100
Quartz Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
Q100-30-7.5 1.00 mm 0.30 mm 7.5 cm 100
Q100-30-15 1.00 mm 0.30 mm 15 cm 100
Q100-50-7.5 1.00 mm 0.50 mm 7.5 cm 100
Q100-50-10 1.00 mm 0.50 mm 10 cm 100
Q100-70-7.5 1.00 mm 0.70 mm 7.5 cm 100
Q100-70-10 1.00 mm 0.70 mm 10 cm 100
Q114-53-10NP* 1.14 mm 0.53 mm 10 cm 100
Q120-40-7.5 1.20 mm 0.40 mm 7.5 cm 100
Q120-60-7.5 1.20 mm 0.60 mm 7.5 cm 100
Q120-90-7.5 1.20 mm 0.90 mm 7.5 cm 100
Q120-90-10 1.20 mm 0.90 mm 10 cm 100
Q150-50-7.5 1.50 mm 0.50 mm 7.5 cm 100
Q150-75-7.5 1.50 mm 0.75 mm 7.5 cm 100
Q150-75-10 1.50 mm 0.75 mm 10 cm 100
Q150-110-10 1.50 mm 1.10 mm 10 cm 100
Q165-115-10 1.65 mm 1.15 mm 10 cm 100
* Nanoinjection glass. The ends are not fire-polished.
Solid Quartz Rod
Catalog Number Style Outside Diameter Overall Length Pieces per Package
QR100-7.5 solid 1.00 mm 7.5 cm 100
QR100-10 solid 1.00 mm 10 cm 100
QR100-15 solid 1.00 mm 15 cm 100
Quartz Theta Glass
Catalog Number Outside Diameter Septum Thickness Overall Length Pieces per Package
QT120-90-7.5 1.20/0.9 mm 0.15 mm 7.5 cm 50
Multibarrel Quartz Glass
Catalog Number Number of Barrels OD/ID in mm Overall Length Pieces per Package
7Q033-16-10 7 barrel 1.00/[.33/.16 ea] 10 cm 50
Aluminosilicate Glass
With Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
AF100-64-10 1.00 mm 0.64 mm 10 cm 100
AF120-77-10 1.20 mm 0.77 mm 10 cm 100
AF120-87-10 1.20 mm 0.87 mm 10 cm 100
AF150-100-10 1.50 mm 1.00 mm 10 cm 100
Aluminosilicate Glass
Without Filament
Catalog Number Outside Diameter Inside Diameter Overall Length Pieces per Package
A100-64-10 1.00 mm 0.64 mm 10 cm 100
A120-77-10 1.20 mm 0.77 mm 10 cm 100
A120-87-10 1.20 mm 0.87 mm 10 cm 100
A150-100-10 1.50 mm 1.00 mm 10 cm 100
Borosilicate Theta Glass
Catalog Number O.D/I.D. Septum Thickness Overall Length Pieces per Package
BT-150-10 1.50/1.17 mm 0.165 mm 10 cm 100
Solid Borosilicate Rod
Catalog Number Style Outside Diameter Overall Length Pieces per Package
BR-100-10 solid 1.00 mm 10 cm 250
BR-100-15 solid 1.00 mm 15 cm 250

 

 

 

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